Course Description

Course Name

Molecular and Microbial Genetics

Session: VDNS3121

Hours & Credits

18 Credit Points

Prerequisites & Language Level

Taught In English

  • There is no language prerequisite for courses at this language level.


Mutations; genetic analysis in bacteria; mobile genetic elements; genetic analysis of regulatory circuits; DNA cloning and sequencing in genetic analysis; comparative microbial genomics; genetics and evolution of viruses of eukaryotes.
GENE 221 provides foundation material on molecular and microbial genetics. Topics include mutation, gene transfer and genetic analysis in bacteria, DNA cloning, and comparative genomics of bacteria, fungi and the HIV retrovirus. Discoveries and methodologies in microbial genetics underlie all aspects of modern biology and therefore this paper is directly relevant for students with an interest in any of the biological sciences. The lecture course is complemented by a laboratory course that includes four experiments that together give hands-on experience of many of the genetic principles that are discussed in the lectures.

Course Structure
The lecture course is divided into 6 modules:
-Mutations - the basis of geneticsGene transfer and genetic analysis in bacteria
-Genetic analysis of regulatory circuits
-Comparative microbial genomics and evolution
-Comparative fungal genomics
-Genetics of HIV

The lecture course is complemented by a laboratory course, which provides training in relevant genetic methods and data analysis, including mutagen detection using the Ames test, gene mapping by conjugation, transposon mutagenesis and DNA cloning using a plasmid vector.

Learning Outcomes
The broad objectives of GENE 221 are to understand:
-The molecular basis and significance of mutation
-Classical genetics of bacteria
-The genetic approaches used in analysing gene regulation in bacteria
-DNA cloning as a tool for genetic analysis
-The use of comparative genomics in understanding the evolution of bacteria, yeast, other fungi and the HIV retrovirus

The recommended text book for GENE 221 is:
Introduction to Genetic Analysis (Griffiths, Wessler, Lewontin, Gelbart, Suzuki and Miller), 10th Edition. The 9th Edition of this book is also satisfactory if you have access to a copy.

*Course content subject to change